A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods

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A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods.

Bactrocera oleae (Rossi) (Diptera: Tephritidae) is considered the most devastating pest of the olive tree worldwide. In an effort to develop management and biological control strategies against this pest, new molecular tools are urgently needed. In this study, we present the design of B. oleae-specific primers based on mitochondrial DNA sequences of cytochrome oxidase subunit I (COI) gene. Two ...

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Role of edaphic arthropods on the biological control of the olive fruit fly (Bactrocera oleae)

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Acetobacter tropicalis is a major symbiont of the olive fruit fly (Bactrocera oleae).

Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with Bactrocera oleae, one of the major agricultural pests in olive-producing countries. Bacterial 16S rRNA gene libraries and ultrastructural analyses revealed the presence of several bacterial taxa associated with this insect, among which Acetobacter tropicalis was predominant. The recent in...

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Computer Design of Novel Pesticides Against the Olive Fruit Fly (Bactrocera oleae)

The olive fruit fly Bactrocera oleae targets the fruits of olive trees in the Mediterranean and has been causing increasing economic harm to Californian growers. Past efforts to combat Bactrocera oleae by limiting the spread of its population have included traps, bait sprays, barrier films, and the release of natural predators, but these methods do little against large populations and many frui...

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Response of olive fruit fly Bactrocera oleae to various attractant combinations, in orchards of Crete

McPhail traps loaded with various attractant solutions (ammonium sulphate, Entomela 75%, Entomela 50%, Dacus bait 100, sexual pheromone of olive fruit fly Bactrocera oleae) combined also with registered plant protection products (lambda-cyhalothrin, alpha-cypermethrin as well as dimethoate) were tested for their attractiveness which was indicated as captures of flies in McPhail traps during thr...

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ژورنال

عنوان ژورنال: Bulletin of Entomological Research

سال: 2016

ISSN: 0007-4853,1475-2670

DOI: 10.1017/s000748531600050x